Brucella canis: Diagnostic tests

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5. Diagnostic tests

Published 5 July 2023 | Updated 5 January 2024

The diagnosis of canine brucellosis is acknowledged to be difficult and is likely to require the combination of results from a number of different methods. In interpreting the results of any test. it is important to be aware of the limitations of the test methodology and the effect of disease prevalence on the predictive value of the results. It is also important to be aware that new tests are being developed and to check with individual laboratories regarding the details of the exact tests that they are using.

This section provides references to a selection of papers on different test methodologies and  the understanding and interpretation of test results.

  • Keid, L.B. et al. (2009) Comparison of agar gel immunodiffusion test, rapid slide agglutination test, microbiological culture and PCR for the diagnosis of canine brucellosis. Research in Veterinary Science, 86 (1), pp. 22-26.
  • Kurmanov, B. et al. (2022) Assays for identification and differentiation of Brucella species: A review. Microorganisms, 10 (8), no.1584.
  • Mol, J.P. et al. (2020) Diagnosis of canine brucellosis: comparison of various serologic tests and PCR. Journal of Veterinary Diagnostic Investigation, 32 (1), pp. 77-86.
  • Keid, L.B. et al. (2010) Comparison of a PCR assay in whole blood and serum specimens for canine brucellosis diagnosis. Veterinary Record, 167 (3), pp. 96-99.
  • Kauffman, L.K. et al. (2014) Early detection of Brucella canis via quantitative polymerase chain reaction analysis. Zoonoses and Public Health, 61 (1), pp. 48-54.
  • da Silva, D.A.V. et al. (2020) MALDI-TOF MS and genomic analysis can make the difference in the clarification of canine brucellosis outbreaks. Scientific Reports, 10 (1), no. 19246.
  • Keid, L. et al. (2015) Evaluation of an immunochromatographic test to the diagnosis of canine brucellosis caused by Brucella canis. Reproduction of Domestic Animals, 50 (6), pp. 939-944.
  • De Oliveira, M.Z.D. et al. (2011) Validation of an ELISA method for the serological diagnosis of canine brucellosis due to Brucella canisResearch in Veterinary Science, 90 (3), pp. 425-431.
  • Yang, Y. et al. (2017) Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay. Scientific Reports, 7(1), no. 1067.
  • Silva Andrade, R. et al. (2023) Accuracy of serological tests for bovine brucellosis: a systematic review and meta-analysis. Preventive Veterinary Medicine, 222, no. 106079.

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