Liquid biopsy in cancer – Molecular, genetic and genomic biomarkers – Cell free DNA and next generation sequencing

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Molecular, genetic and genomic biomarkers

Cell free DNA and next generation sequencing

Published 29 May 2025

These tests use genetic sequencing to look for changes in the cell free DNA such as mutations, including single nucleotide polymorphism (SNP) copy number variations (CNV), which may be associated with particular types of cancer.

  • Tagawa, M. et al. (2024) Clinical utility of circulating cell‐free DNA as a liquid biopsy in cats with various tumours.Veterinary and Comparative Oncology, 22 (4), pp. 592-601. https://doi.org/10.1111/vco.13013
  • Kim, J. et al. (2021) Cell-free DNA as a diagnostic and prognostic biomarker in dogs with tumors. Frontiers in Veterinary Science, 8. https://doi.org/10.3389/fvets.2021.735682
  • Kruglyak, K.M. et al. (2021) Blood-based liquid biopsy for comprehensive cancer genomic profiling using next-generation sequencing: An emerging paradigm for non-invasive cancer detection and management in dogs.Frontiers in Veterinary Science, 8, no. 704835. https://doi.org/10.3389/fvets.2021.704835
  • McCleary-Wheeler, A.L. et al. (2024) Next-generation sequencing-based liquid biopsy may be used for detection of residual disease and cancer recurrence monitoring in dogs. American Journal of Veterinary Research, 85 (3). https://doi.org/10.2460/ajvr.23.07.0163
  • Ruiz-Perez, C.A. et al. (2024) Proof-of-concept evaluation of next-generation sequencing-based liquid biopsy for non-invasive cancer detection in cats. Frontiers in Veterinary Science, 11. https://doi.org/10.3389/fvets.2024.1394686

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